Composite

Part:BBa_K5335013:Design

Designed by: Beier Tu   Group: iGEM24_HZAU-China   (2024-09-27)


This part includes holin and PGHs.holin is a type of perforin, and PGHs is a phage-encoded peptidogl


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 907
    Illegal XbaI site found at 101
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 907
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 907
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 907
    Illegal XbaI site found at 101
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 907
    Illegal XbaI site found at 101
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Note that the distance between RBS and the start codon is appropriate.


Source

Holin and peptidoglycan hydrolase are derived from Phage VB_sup-GOe1.Two RBS come from the iGEM parts library.The DNA sequence was optimized and synthesized by Genscript.

References